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An orange fluorescent protein with a large Stokes shift for single-excitation multicolor FCCS and FRET imaging

Multicolor imaging based on genetically-encoded fluorescent proteins (FPs) is a powerful approach to study several dynamic processes in a live cell. We report a monomeric orange FP with a large Stokes shift (LSS), called LSSmOrange (excitation/emission at 437/572 nm), which fills up an existing spec...

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Bibliographic Details
Main Authors: Shcherbakova, Daria M., Hink, Mark A., Joosen, Linda, Gadella, Theodorus W. J., Verkhusha, Vladislav V.
Format: Artigo
Language:Inglês
Published: 2012
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3348967/
https://ncbi.nlm.nih.gov/pubmed/22486524
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja3018972
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