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Sensitive non-radioactive dot-blot hybridization using DNA probes labelled with chelate group substituted psoralen and quantitative detection by europium ion fluorescence.

A new labelling method for cloned DNA probes used in hybridization assays is described. The DNA insert of recombinant plasmid DNA was made partially single-stranded for the labelling reaction by a restriction enzyme digest, followed by a controlled exonuclease III incubation. A thiol-containing psor...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Oser, A, Roth, W K, Valet, G
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1988
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC334746/
https://ncbi.nlm.nih.gov/pubmed/3344204
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