5'-3' exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis.
The application of T7 and lambda exonuclease to phosphorothioate-based oligonucleotide-directed mutagenesis was investigated. Oligonucleotide primers designed to introduce single or double base mismatches, an insertion or a deletion (each of 16 bases) were annealed to M13 phage derivatives. Double s...
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| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
1988
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| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334719/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2830593/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.3.791 |
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