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Mutations Derived from the Thermophilic Polyhydroxyalkanoate Synthase PhaC Enhance the Thermostability and Activity of PhaC from Cupriavidus necator H16

The thermophile Cupriavidus sp. strain S-6 accumulated polyhydroxybutyrate (PHB) from glucose at 50°C. A 9.0-kbp EcoRI fragment cloned from the genomic DNA of Cupriavidus sp. S-6 enabled Escherichia coli XL1-Blue to synthesize PHB at 45°C. Nucleotide sequence analysis showed a pha locus in the clone...

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Main Authors: Sheu, Der-Shyan, Chen, Wen-Ming, Lai, Yung-Wei, Chang, Rey-Chang
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2012
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3347165/
https://ncbi.nlm.nih.gov/pubmed/22408158
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.06543-11
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