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Rapid and Sensitive Detection of Mycobacterium ulcerans by Use of a Loop-Mediated Isothermal Amplification Test

This work reports the design and evaluation of a rapid loop-mediated isothermal amplification test for detecting Mycobacterium ulcerans DNA based on the multicopy insertion sequence IS2404. The test is robust and specific with a detection limit equivalent to 20 copies of the target sequence (0.01 to...

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Bibliographic Details
Main Authors: Njiru, Zablon K., Yeboah-Manu, Dorothy, Stinear, Timothy P., Fyfe, Janet A. M.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2012
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3347114/
https://ncbi.nlm.nih.gov/pubmed/22357495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.06460-11
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