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Real-Time PCR Assay for Identification of Blastomyces dermatitidis in Culture and in Tissue

The TaqMan real-time PCR assay was developed from the Blastomyces dermatitidis BAD1 gene promoter. The assay identified all haplotypes of B. dermatitidis and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast f...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Sidamonidze, Ketevan, Peck, Marcia K., Perez, Michael, Baumgardner, Dennis, Smith, Gerald, Chaturvedi, Vishnu, Chaturvedi, Sudha
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: American Society for Microbiology 2012
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3347106/
https://ncbi.nlm.nih.gov/pubmed/22403418
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00310-12
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