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Real-Time PCR Assay for Identification of Blastomyces dermatitidis in Culture and in Tissue
The TaqMan real-time PCR assay was developed from the Blastomyces dermatitidis BAD1 gene promoter. The assay identified all haplotypes of B. dermatitidis and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast f...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology
2012
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3347106/ https://ncbi.nlm.nih.gov/pubmed/22403418 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00310-12 |
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