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Versatile Transformation System That Is Applicable to both Multiple Transgene Expression and Gene Targeting for Thraustochytrids

A versatile transformation system for thraustochytrids, a promising producer for polyunsaturated fatty acids and fatty acid-derived fuels, was established. G418, hygromycin B, blasticidin, and zeocin inhibited the growth of thraustochytrids, indicating that multiple selectable marker genes could be...

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Detalhes bibliográficos
Main Authors: Sakaguchi, Keishi, Matsuda, Takanori, Kobayashi, Takumi, Ohara, Jun-ichiro, Hamaguchi, Rie, Abe, Eriko, Nagano, Naoki, Hayashi, Masahiro, Ueda, Mayumi, Honda, Daiske, Okita, Yuji, Taoka, Yousuke, Sugimoto, Shinichi, Okino, Nozomu, Ito, Makoto
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3346472/
https://ncbi.nlm.nih.gov/pubmed/22344656
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.07129-11
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