The N-Terminal β-Sheet of the Hyperthermophilic Endoglucanase from Pyrococcus horikoshii Is Critical for Thermostability

The β-1,4-endoglucanase (EC 3.2.1.4) from the hyperthermophilic archaeon Pyrococcus horikoshii (EGPh) has strong hydrolyzing activity toward crystalline cellulose. When EGPh is used in combination with β-glucosidase (EC 3.2.1.21), cellulose is completely hydrolyzed to glucose at high temperature, su...

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Detalhes bibliográficos
Main Authors: Yang, Trent C., Legault, Steve, Kayiranga, Emery A., Kumaran, Jyothi, Ishikawa, Kazuhiko, Sung, Wing L.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2012
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3346460/
https://ncbi.nlm.nih.gov/pubmed/22344652
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.07576-11
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