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New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based Counterselection System for Generating Mutants by Allelic Replacement in Staphylococcus aureus

We have developed a range of vectors for allelic replacements in Staphylococcus aureus to facilitate genetic work in this opportunistic pathogen. The central feature of the vector range is a selection/counterselection system that takes advantage of the 5-fluoroorotic acid (FOA) resistance and pyrimi...

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Bibliografski detalji
Glavni autori: Redder, Peter, Linder, Patrick
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2012
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3346405/
https://ncbi.nlm.nih.gov/pubmed/22447609
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00202-12
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