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Identification of the origin and direction of replication of the broad-host-range plasmid pLS1.

The replication origin of the fully sequenced broad-host-range streptococcal plasmid pLS1 has been determined by the use of an in vitro replication system prepared from Escherichia coli, a host in which the plasmid can be established. Replicative intermediates were isolated from reaction mixtures th...

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Bibliographic Details
Main Authors: Puyet, A, del Solar, G H, Espinosa, M
Format: Artigo
Language:Inglês
Published: 1988
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC334616/
https://ncbi.nlm.nih.gov/pubmed/2829114
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