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Purification and properties of the Eco57I restriction endonuclease and methylase--prototypes of a new class (type IV).

The Eco57I restriction endonuclease and methylase were purified to homogeneity from the E.coli RR1 strain carrying the eco57IRM genes on a recombinant plasmid. The molecular weight of the denaturated methylase is 63 kDa. The restriction endonuclease exists in a monomeric form with an apparent molecu...

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Autors principals: Janulaitis, A, Petrusyte, M, Maneliene, Z, Klimasauskas, S, Butkus, V
Format: Artigo
Idioma:Inglês
Publicat: 1992
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC334471/
https://ncbi.nlm.nih.gov/pubmed/1334260
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