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Analysis of spliceosome assembly and the structure of a regulated intron in Drosophila in vitro splicing extracts.

We characterize spliceosome assembly in Drosophila embryonic nuclear extracts. Further, we show that these extracts contain high levels of a 5' to 3' exoribonuclease activity allowing rapid, convenient protection mapping of 5' splice site and branchpoint sequences. We use this assay t...

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Hlavní autoři: Spikes, D, Bingham, P M
Médium: Artigo
Jazyk:Inglês
Vydáno: 1992
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC334408/
https://ncbi.nlm.nih.gov/pubmed/1333586
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