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Analysis of spliceosome assembly and the structure of a regulated intron in Drosophila in vitro splicing extracts.
We characterize spliceosome assembly in Drosophila embryonic nuclear extracts. Further, we show that these extracts contain high levels of a 5' to 3' exoribonuclease activity allowing rapid, convenient protection mapping of 5' splice site and branchpoint sequences. We use this assay t...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1992
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334408/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1333586/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.21.5719 |
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