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The selective isolation of novel cDNAs encoded by the regions surrounding the human interleukin 4 and 5 genes.
We have developed modifications to direct cDNA selection that allow the rapid and reproducible isolation of low abundance cDNAs encoded by large genomic clones. Biotinylated, cloned genomic DNAs are hybridized in solution with amplifiable cDNAs. The genomic clones and attached cDNAs are captured on...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1992
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC334302/ https://ncbi.nlm.nih.gov/pubmed/1408833 |
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