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A rapid chemiluminescent method for quantitation of human DNA.

A sensitive and simple method for the quantitation of human DNA is described. This method is based on probe hybridization to a human alpha satellite locus, D17Z1. The biotinylated probe is hybridized to sample DNA immobilized on nylon membrane. The subsequent binding of streptavidin-horseradish pero...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Walsh, P S, Varlaro, J, Reynolds, R
Format: Artigo
Language:Inglês
Published: Oxford University Press 1992
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334284/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1408822/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.19.5061
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