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Gene rescue in plants by direct gene transfer of total genomic DNA into protoplasts.
To study the possibility of gene rescue in plants by direct gene transfer we chose the Arabidopsis mutant GH50 as a source of donor DNA. GH50 is tolerant of chlorsulfuron, a herbicide of the sulfonylurea class. Tobacco protoplasts were cotransfected with genomic DNA and the plasmid pHP23 which confe...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1992
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC334075/ https://ncbi.nlm.nih.gov/pubmed/1508682 |
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