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Single-molecule imaging of DNA curtains reveals mechanisms of KOPS sequence targeting by the DNA translocase FtsK

FtsK is a hexameric DNA translocase that participates in the final stages of bacterial chromosome segregation. Here we investigate the DNA-binding and translocation activities of FtsK in real time by imaging fluorescently tagged proteins on nanofabricated curtains of DNA. We show that FtsK preferent...

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Main Authors: Lee, Ja Yil, Finkelstein, Ilya J., Crozat, Estelle, Sherratt, David J., Greene, Eric C.
Formáid: Artigo
Teanga:Inglês
Foilsithe: National Academy of Sciences 2012
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3340036/
https://ncbi.nlm.nih.gov/pubmed/22493241
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1201613109
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