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Ribonuclease T1 generates circular RNA molecules from viroid-specific RNA transcripts by cleavage and intramolecular ligation.

A 406 nucleotide long potato spindle tuber viroid (PSTVd)-specific linear RNA transcript was synthesized in vitro and subjected to limited digestion with ribonuclease (RNase) T1. Under certain conditions this guanosine-specific endoribonuclease proved to be capable of processing the longer-than-unit...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Tsagris, M, Tabler, M, Sänger, H L
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 1991
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC333922/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1709278/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.7.1605
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