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Ultra-Rapid Warming Yields High Survival of Mouse Oocytes Cooled to −196°C in Dilutions of a Standard Vitrification Solution

Intracellular ice is generally lethal. One way to avoid it is to vitrify cells; that is, to convert cell water to a glass rather than to ice. The belief has been that this requires both the cooling rate and the concentration of glass-inducing solutes be very high. But high solute concentrations can...

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Autori principali: Seki, Shinsuke, Mazur, Peter
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3338624/
https://ncbi.nlm.nih.gov/pubmed/22558325
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0036058
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