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Optimized Expression and Purification for High-Activity Preparations of Algal [FeFe]-Hydrogenase
BACKGROUND: Recombinant expression and purification of metallo-enzymes, including hydrogenases, at high-yields is challenging due to complex, and enzyme specific, post-translational maturation processes. Low fidelities of maturation result in preparations containing a significant fraction of inactiv...
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| Hlavní autoři: | , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Public Library of Science
2012
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3338550/ https://ncbi.nlm.nih.gov/pubmed/22563413 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0035886 |
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