A simple method for construction of pir(+ )Enterobacterial hosts for maintenance of R6K replicon plasmids

BACKGROUND: The R6K replicon is one of the best studied bacterial plasmid replicons. Replication of the R6K plasmid and derivatives harboring its γ origin of replication (ori(R6Kγ)) is dependent on the pir gene-encoded π protein. Originally encoded by R6K, this protein is usually provided in trans i...

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Detalhes bibliográficos
Main Authors: Kvitko, Brian H, Bruckbauer, Steven, Prucha, John, McMillan, Ian, Breland, Erin J, Lehman, Stephanie, Mladinich, Katie, Choi, Kyoung-Hee, Karkhoff-Schweizer, RoxAnn, Schweizer, Herbert P
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
Assuntos:
Technical Note
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3338088/
https://ncbi.nlm.nih.gov/pubmed/22433797
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-5-157
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