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Enzymatic cleavage of a bacterial chromosome at a transposon-inserted rare site.
The sequential use of the methylase M.Xbal (5'.TCTAGm6A) and the methylation-dependent endonuclease Dpnl (5'-Gm6A decreases TC) results in cleavage at 5'.TCTAGA decreases TCTAGA. This recognition sequence was introduced into a transposon derived from the Mu bacteriophage and transpose...
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| Auteurs principaux: | , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
1991
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| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC333718/ https://ncbi.nlm.nih.gov/pubmed/1850125 |
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