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Isolation and stability of ternary complexes of elongation factor Tu, GTP and aminoacyl-tRNA.

Intact, native EF-Tu, isolated using previously described methods and fully active in binding GTP, was never found to be fully active in binding aminoacyl-tRNA as judged by high performance liquid chromatography (HPLC) gel filtration and zone-interference gel-electrophoresis. In the presence of kirr...

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Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Abrahams, J P, Kraal, B, Clark, B F, Bosch, L
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 1991
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC333647/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2011527/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.3.553
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