Isolation and stability of ternary complexes of elongation factor Tu, GTP and aminoacyl-tRNA.
Intact, native EF-Tu, isolated using previously described methods and fully active in binding GTP, was never found to be fully active in binding aminoacyl-tRNA as judged by high performance liquid chromatography (HPLC) gel filtration and zone-interference gel-electrophoresis. In the presence of kirr...
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| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1991
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC333647/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2011527/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.3.553 |
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