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High-sensitivity two-color detection of double-stranded DNA with a confocal fluorescence gel scanner using ethidium homodimer and thiazole orange.

Ethidium homodimer (EthD; lambda Fmax 620 nm) at EthD:DNA ratios up to 1 dye:4-5 bp forms stable fluorescent complexes with double-stranded DNA (dsDNA) which can be detected with high sensitivity using a confocal fluorescence gel scanner (Glazer, A.N., Peck, K. & Mathies, R.A. (1990) Proc. Natl....

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Enregistré dans:
Détails bibliographiques
Auteurs principaux: Rye, H S, Quesada, M A, Peck, K, Mathies, R A, Glazer, A N
Format: Artigo
Langue:Inglês
Publié: 1991
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC333598/
https://ncbi.nlm.nih.gov/pubmed/2014172
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