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Post-PCR sterilization: a method to control carryover contamination for the polymerase chain reaction.

We describe a photochemical procedure for the sterilization of polynucleotides that are created by the Polymerase Chain Reaction (PCR). The procedure is based upon the blockage of Taq DNA polymerase when it encounters a photochemically modified base in a polynucleotide strand. We have discovered rea...

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Bibliografski detalji
Glavni autori: Cimino, G D, Metchette, K C, Tessman, J W, Hearst, J E, Isaacs, S T
Format: Artigo
Jezik:Inglês
Izdano: 1991
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC333539/
https://ncbi.nlm.nih.gov/pubmed/2011516
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