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miR-Sens—a retroviral dual-luciferase reporter to detect microRNA activity in primary cells
MicroRNA–mRNA interactions are commonly validated and deconstructed in cell lines transfected with luciferase reporters. However, due to cell type-specific variations in microRNA or RNA-binding protein abundance, such assays may not reliably reflect microRNA activity in other cell types that are les...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3334695/ https://ncbi.nlm.nih.gov/pubmed/22417692 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.031831.111 |
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