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Normalized Affymetrix expression data are biased by G-quadruplex formation

Probes with runs of four or more guanines (G-stacks) in their sequences can exhibit a level of hybridization that is unrelated to the expression levels of the mRNA that they are intended to measure. This is most likely caused by the formation of G-quadruplexes, where inter-probe guanines form Hoogst...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Shanahan, Hugh P., Memon, Farhat N., Upton, Graham J. G., Harrison, Andrew P.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3333884/
https://ncbi.nlm.nih.gov/pubmed/22199258
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1230
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