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Analysis of alternative splicing of cassette exons at single-cell level using two fluorescent proteins

Alternative splicing plays a major role in increasing proteome complexity and regulating gene expression. Here, we developed a new fluorescent protein-based approach to quantitatively analyze the alternative splicing of a target cassette exon (skipping or inclusion), which results in an open-reading...

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Autors principals: Gurskaya, Nadya G., Staroverov, Dmitry B., Zhang, Lijuan, Fradkov, Arkady F., Markina, Nadezhda M., Pereverzev, Anton P., Lukyanov, Konstantin A.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2012
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3333876/
https://ncbi.nlm.nih.gov/pubmed/22259036
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1314
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