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Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)

Urea PAGE or denaturing urea polyacrylamide gel electrophoresis employs 6-8 M urea, which denatures secondary DNA or RNA structures and is used for their separation in a polyacrylamide gel matrix based on the molecular weight. Fragments between 2 to 500 bases, with length differences as small as a s...

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Detalhes bibliográficos
Main Authors: Summer, Heike, Grämer, René, Dröge, Peter
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3329804/
https://ncbi.nlm.nih.gov/pubmed/19865070
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1485
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