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A Redox-Sensitive Luciferase Assay for Determining the Localization and Topology of Endoplasmic Reticulum Proteins

Correct localization and transmembrane topology are crucial for the proteins residing and functioning in the endoplasmic reticulum (ER). We have developed a rapid and convenient assay, based on the redox-sensitive luciferase from Gaussia princeps (Gluc) and green fluorescence protein (GFP), to deter...

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Detalles Bibliográficos
Autores principales: Li, Hai-Yin, Zheng, Xue-Ming, Che, Mei-Xia, Hu, Hong-Yu
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science 2012
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3329452/
https://ncbi.nlm.nih.gov/pubmed/22530060
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0035628
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