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A single centrifugation method for isolating fat droplets from cells and tissues

Fat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tissue are fractionated with a single centrifugation...

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Bibliografiska uppgifter
Huvudupphovsmän: Harris, Lydia-Ann L. S., Shew, Trevor M., Skinner, James R., Wolins, Nathan E.
Materialtyp: Artigo
Språk:Inglês
Publicerad: The American Society for Biochemistry and Molecular Biology 2012
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3329379/
https://ncbi.nlm.nih.gov/pubmed/22327205
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D023598
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