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A single centrifugation method for isolating fat droplets from cells and tissues

Fat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tissue are fractionated with a single centrifugation...

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Detalles Bibliográficos
Autores principales: Harris, Lydia-Ann L. S., Shew, Trevor M., Skinner, James R., Wolins, Nathan E.
Formato: Artigo
Lenguaje:Inglês
Publicado: The American Society for Biochemistry and Molecular Biology 2012
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3329379/
https://ncbi.nlm.nih.gov/pubmed/22327205
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D023598
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