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Promoter selectivity of Escherichia coli RNA polymerase: effect of base substitutions in the promoter -35 region on promoter strength.

A set of 18 variant lac UV5 promoters was constructed, each carrying a single base substitution within the -35 region (nucleotide positions from -36 to -31 relative to the transcription start site). Using truncated DNA fragments carrying these variant promoters and purified Escherichia coli RNA poly...

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Bibliographic Details
Main Authors: Kobayashi, M, Nagata, K, Ishihama, A
Format: Artigo
Language:Inglês
Published: 1990
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC332874/
https://ncbi.nlm.nih.gov/pubmed/2259628
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