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Frequency-Selective Heteronuclear Dephasing and Selective Carbonyl Labeling to Deconvolute Crowded Spectra of Membrane Proteins By Magic Angle Spinning NMR

We present a new method that combines carbonyl-selective labeling with frequency-selective heteronuclear recoupling to resolve the spectral overlap of magic angle spinning (MAS) NMR spectra of membrane proteins in fluid lipid membranes with broad lines and high redundancy in the primary sequence. We...

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Détails bibliographiques
Auteurs principaux: Traaseth, Nathaniel J., Veglia, Gianluigi
Format: Artigo
Langue:Inglês
Publié: 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3328402/
https://ncbi.nlm.nih.gov/pubmed/21482162
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmr.2011.03.013
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