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An easy and versatile 2-step protocol for targeted modification and subcloning of DNA from bacterial artificial chromosomes using non-commercial plasmids
BACKGROUND: Promoter-specific expression of foreign DNA in transgenic organisms often relies on bacterial artificial chromosomes (BACs). This approach requires modification and subcloning of BAC-DNA by recombineering technologies in Escherichia coli. Most current protocols rely on commercial kits or...
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| Huvudupphovsmän: | , |
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| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
BioMed Central
2012
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| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3327625/ https://ncbi.nlm.nih.gov/pubmed/22433714 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-5-156 |
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