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Purification of polyhydroxybutyrate synthase from its native organism, Ralstonia eutropha: implications in the initiation and elongation of polymer formation in vivo

Class I polyhydroxybutyrate (PHB) synthase (PhaC) from Ralstonia eutropha catalyzes the formation of PHB from (R)-3-hydroxybutyryl-CoA, ultimately resulting in the formation of insoluble granules. Previous mechanistic studies of R. eutropha PhaC, purified from Escherichia coli (PhaC(Ec)), demonstrat...

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Autori principali: Cho, Mimi, Brigham, Christopher J., Sinskey, Anthony J., Stubbe, JoAnne
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3326396/
https://ncbi.nlm.nih.gov/pubmed/22369488
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi2013596
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