Derepression of a mouse alpha-fetoprotein expression vector in COS-1 cells by amplification of specific cis-acting sequences of the AFP promoter.
The existence of trans-acting regulatory factors has been demonstrated by in vivo competition with cis-acting sequences from both viral and eukaryotic genomes. Plasmids containing a functional SV40 origin of replication when transfected into permissive SV40 T-antigen producing COS-1 cells will ampli...
Gespeichert in:
| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1990
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC332628/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1701243/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.22.6677 |
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