High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe.

We describe a highly efficient alkali cation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of fission yeast Schizosaccharomyces pombe mutants. cDNA libraries constructed with the pcD or pcD2 vector are transduced into yeast by cotransfection with a linea...

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Bibliografski detalji
Glavni autori: Okazaki, K, Okazaki, N, Kume, K, Jinno, S, Tanaka, K, Okayama, H
Format: Artigo
Jezik:Inglês
Izdano: 1990
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC332599/
https://ncbi.nlm.nih.gov/pubmed/2251111
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