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A highly efficient directional cDNA cloning method utilizing an asymmetrically tailed linker-primer plasmid.
A new procedure using an asymmetrically tailed linker-primer plasmid has been developed to prepare extremely high complexity cDNA libraries. This procedure yields plasmid primed libraries with a final form equivalent to those made by the procedure of Okayama and Berg. However, the number of steps in...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1991
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC332525/ https://ncbi.nlm.nih.gov/pubmed/1766871 |
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