Subtractive cDNA cloning using oligo(dT)30-latex and PCR: isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells.

The human embryonal carcinoma cell line NEC14 can be induced to differentiate by the addition of 10(-2)M N,N'-hexamethylene-bis-acetamide (HMBA). A subtractive cDNA library specific to undifferentiated NEC14 cells was constructed using oligo(dT)30-Latex and polymerase chain reaction (PCR). The metho...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Hara, E, Kato, T, Nakada, S, Sekiya, S, Oda, K
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1991
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC332523/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1766870/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.25.7097
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