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A New Generation of T7 RNA Polymerase-Independent Inducible Expression Plasmids for Trypanosoma brucei

Expression of transgenes is central to forward and reverse genetic analysis in Trypanosoma brucei. The inducible expression of transgenes in trypanosomes is based on the tetracycline repressor binding to a tetracycline operator to prevent transcription in the absence of tetracycline. The same induci...

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Detalhes bibliográficos
Main Authors: Sunter, Jack, Wickstead, Bill, Gull, Keith, Carrington, Mark
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3325195/
https://ncbi.nlm.nih.gov/pubmed/22511983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0035167
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