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A New Generation of T7 RNA Polymerase-Independent Inducible Expression Plasmids for Trypanosoma brucei

Expression of transgenes is central to forward and reverse genetic analysis in Trypanosoma brucei. The inducible expression of transgenes in trypanosomes is based on the tetracycline repressor binding to a tetracycline operator to prevent transcription in the absence of tetracycline. The same induci...

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Autors principals: Sunter, Jack, Wickstead, Bill, Gull, Keith, Carrington, Mark
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3325195/
https://ncbi.nlm.nih.gov/pubmed/22511983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0035167
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