Construction of a human chromosome 3 specific NotI linking library using a novel cloning procedure.
Two new diphasmid vectors (lambda SK17 and SK22) and a novel procedure to construct linking libraries are described. A partial filling-in reaction provides counter-selection against false linking clones in the library, and obviates the need for supF selection. The diphasmid vectors, in combination w...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , , , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1990
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC332498/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2243778/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.21.6319 |
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