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Selection for mutations in the PR promoter of bacteriophage lambda.

Insertion of DNA containing PR, the early rightward promoter of bacteriophage lambda, is lethal to M13-derived vectors when the promoter directs transcription (using the '+' strand as template) toward the M13 origin of replication (ori). Lethality can be relieved by mutation of PR, repress...

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Bibliographic Details
Main Authors: Brown, S, Ferm, J, Woody, S, Gussin, G
Format: Artigo
Language:Inglês
Published: 1990
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC332391/
https://ncbi.nlm.nih.gov/pubmed/2146590
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