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Thermodynamics of triple helix formation: spectrophotometric studies on the d(A)10.2d(T)10 and d(C+3T4C+3).d(G3A4G3).d(C3T4C3) triple helices.

We have stabilized the d(A)10.2d(T)10 and d(C+LT4C+3).d(G3A4G3).d(C3T4C3) triple helices with either NaCl or MgCl2 at pH 5.5. UV mixing curves demonstrate a 1:2 stoichiometry of purine to pyrimidine strands under the appropriate conditions of pH and ionic strength. Circular dichroic titrations sugge...

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Dades bibliogràfiques
Publicat a:Nucleic Acids Res
Autors principals: Pilch, D S, Brousseau, R, Shafer, R H
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1990
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC332309/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2216768/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.19.5743
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