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Direct detection and automated sequencing of individual alleles after electrophoretic strand separation: identification of a common nonsense mutation in exon 9 of the human lipoprotein lipase gene.

Large-scale screening by direct sequencing of DNA to detect molecular variants remains a laborious endeavor whose difficulty is compounded by heterozygosity. We show that mobility shifts of single-stranded DNA electrophoresed under nondenaturing conditions can be used not only to detect variants (Or...

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Main Authors: Hata, A, Robertson, M, Emi, M, Lalouel, J M
格式: Artigo
語言:Inglês
出版: 1990
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC332217/
https://ncbi.nlm.nih.gov/pubmed/2216713
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