Removal of double-stranded contaminants from RNA transcripts: synthesis of adenovirus VA RNAI from a T7 vector.

Bacteriophage RNA polymerases are widely used to synthesize defined RNAs on a large scale in vitro. Unfortunately, the RNA product contains a small proportion of contaminating RNAs, including complementary species, which can lead to errors of interpretation. We cloned the gene encoding Ad2 VA RNAI i...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Mellits, K H, Pe'ery, T, Manche, L, Robertson, H D, Mathews, M B
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1990
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC332216/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2216712/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.18.5401
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