Dual probe DNA capture for sensitive real-time PCR detection of Cryptosporidium and Giardia

Nucleic acid amplification for the enteropathogens Cryptosporidium and Giardia is complicated by low target template concentrations and PCR inhibitors. In this work we designed dual capture oligonucleotides for both Cryptosporidium and Giardia 18S rRNA targets which when utilized during DNA extracti...

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Bibliografiska uppgifter
Huvudupphovsmän: Stroup, Suzanne, Tongjai, Siripong, Swai, Ndealilia, Maro, Athanasia, Kibiki, Gibson, Houpt, Eric R.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2011
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Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3319247/
https://ncbi.nlm.nih.gov/pubmed/22227113
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mcp.2011.12.003
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