Effects of Decontamination, DNA Extraction, and Amplification Procedures on the Molecular Diagnosis of Mycobacterium ulcerans Disease (Buruli Ulcer)

We compared two DNA extraction methods (a semiautomated method using a Maxwell kit and a modified Boom method) and three amplification procedures (a single-step PCR, a nested PCR, and a real-time quantitative PCR) on 74 surgical tissue specimens from patients with clinically suspected Buruli ulcer....

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Autors principals: Affolabi, Dissou, Sanoussi, N′Dira, Vandelannoote, Koen, Odoun, Mathieu, Faïhun, Frank, Sopoh, Ghislain, Anagonou, Séverin, Portaels, Françoise, Eddyani, Miriam
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2012
Matèries:
Mycobacteriology and Aerobic Actinomycetes
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3318535/
https://ncbi.nlm.nih.gov/pubmed/22259213
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.05592-11
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