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Second-Site Suppression of RNase E Essentiality by Mutation of the deaD RNA Helicase in Escherichia coli
Escherichia coli cells normally require RNase E activity to propagate and form colonies. Using random Tn10 insertion mutagenesis, we screened for second-site suppressor mutations that restore colony-forming ability (CFA) to E. coli cells lacking RNase E function and found mutations in three separate...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology
2012
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3318467/ https://ncbi.nlm.nih.gov/pubmed/22328678 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.06652-11 |
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