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Cell-type-specific nuclei purification from whole animals for genome-wide expression and chromatin profiling

An understanding of developmental processes requires knowledge of transcriptional and epigenetic landscapes at the level of tissues and ultimately individual cells. However, obtaining tissue- or cell-type-specific expression and chromatin profiles for animals has been challenging. Here we describe a...

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Hlavní autoři: Steiner, Florian A., Talbert, Paul B., Kasinathan, Sivakanthan, Deal, Roger B., Henikoff, Steven
Médium: Artigo
Jazyk:Inglês
Vydáno: Cold Spring Harbor Laboratory Press 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3317158/
https://ncbi.nlm.nih.gov/pubmed/22219512
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.131748.111
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