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Chemical Clearing and Dehydration of GFP Expressing Mouse Brains

Generally, chemical tissue clearing is performed by a solution consisting of two parts benzyl benzoate and one part benzyl alcohol. However, prolonged exposure to this mixture markedly reduces the fluorescence of GFP expressing specimens, so that one has to compromise between clearing quality and fl...

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Bibliografiske detaljer
Main Authors: Becker, Klaus, Jährling, Nina, Saghafi, Saiedeh, Weiler, Reto, Dodt, Hans-Ulrich
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3316521/
https://ncbi.nlm.nih.gov/pubmed/22479475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0033916
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