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An improved method for photofootprinting yeast genes in vivo using Taq polymerase.
We have developed an improved method for photofootprinting in vivo which utilizes the thermostable DNA polymerase from T. aquaticus (Taq) in a primer extension assay. UV light is used to introduce photoproducts into the genomic DNA of intact yeast cells. The photoproducts are then detected and mappe...
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| Autori principali: | , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
1989
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| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC331543/ https://ncbi.nlm.nih.gov/pubmed/2643080 |
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